The woman died when her bike collided with an articulated truck at the junction of Rutland Street, Fr. Matthew Bridge and Charlotte's Quay at around 7.30 this morning.
Believed to be in her 30's, she was pronounced dead at the scene.
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The stretch of road – close to The Hunt Museum – is currently closed while Gardaí carry out a forensic examination of the scene.
Diversions are in place.